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Adaptive immune response against BK polyomavirus infection
Rezlerová, Adéla ; Saláková, Martina (advisor) ; Roubalová, Kateřina (referee)
BK polyomavirus is a small non-enveloped virus that is found in a large proportion of the human population. BKPyV infection commonly occurs in early childhood. The virus establishes persistent infection in renal tubular cells and uroepithelial cells. In immunosuppressed individuals, especially after renal or haematopoietic stem cell transplantation, BKPyV reactivation can lead to serious complications such as BKPyV-associated nephropathy (BKVAN), urethral stenosis or haemorrhagic cystitis. Adaptive immunity plays a crucial role in controlling the replication and progression of BKPyV infection. The T cell response is particularly important, with the production of CD4+ and CD8+ T-lymphocytes. B-lymphocytes, which produce virus neutralising antibodies, are also important. Currently, there are no effective antiviral agents against BKPyV infection and reducing immunosuppression remains the main strategy to suppress reactivation. Exploration of immune- based therapies offers promising possibilities for effective treatment of complications associated with polyomavirus infection. Key words: BK polyomavirus, T cell response, antibodies, BK polyomavirus-associated nephropathy, hemorrhagic cystitis
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Subpopulations of lymphocytes in patients with prostate cancer during the immunotherapy by dendritic cell vaccine
Volmová, Martina ; Lašťovička, Jan (advisor) ; Palich Fučíková, Jitka (referee)
The bachelor thesis looks into the issue of cancer immunotherapy and it deals with possible use of immunotherapy by dendritic cell vaccine in patients with prostate cancer, which is now in phase I/II of clinical trials on the Department of Immunology at Faculty hospital Motol. The target of the practical part was to cope with the technology of preparation of blood samples and their measurement by means of the flow cytometry, with subsequent data evaluation and processing. The highlight of this work was the statistical evaluation of obtained data of lymphocyte subpopulations levels in peripheral blood, and their possible correlation with disease progression. The main subject of the research were subpopulations CD3 +, CD4 +, CD8+ , CD16+ , CD19+ and HLA-DR+. The monitoring of these subpopulations in patients treated by dendritic cell vaccine showed the decrease of both leukocyte and lymphocyte levels, reduction of CD4/CD8 index, decrease of relative and absolute numbers of CD4+ cells and significant decrease of both relative and absolute B lymphocyte numbers during the progress of the disease. Powered by TCPDF (www.tcpdf.org)
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